Escherichia coli DH5-alpha-pD25466-pBACpAK-COL-FM1-C2-10-1-SUB- NEB |
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Putative Escherichia coli DH5-alpha-pD25466-pBACpAK-COL-FM1-C2-10-1-SUB- NEB RM systems
Ref: Goodman,R.N. et al. J Microbiol Methods 213, 106813 (2023)
REBASE ref # 37506
| Sequence length: | GenBank #: | |
| Chromosome: | 4,582,781 bp | CP125687 |
| plasmid pD25466: | 115,313 bp | CP125685 |
REBASE acronym: EcoNEB
Org_num: 71613
All begin QL123_
| Type I | ||||
|---|---|---|---|---|
| ORF | Gene | Most similar | Specificity | Name |
| Chromosome | ||||
| 16590 | endoribonuclease SymE | |||
| 16595 | S | S.SenHNK130ORF17125P (100% identity) | AACNNNNNNGTGC | S.EcoNEBORF16600P |
| 16600 | M | M.SenHNK130ORF17125P (100% identity) | AACNNNNNNGTGC | M.EcoNEBORF16600P |
| 16605 | R | Esp17TGCORF18925P (100% identity) | AACNNNNNNGTGC | EcoNEBORF16600P |
| 16610 | adenine/cytosine-specific restriction | |||
| Type II | ||||
| ORF | Gene | Most similar | Specificity | Name |
| Chromosome | ||||
| 4430 | drug/metabolite exporter YedA | |||
| 4435 | V | V.SflFF64DcmP (100% identity) | CCWGG | V.EcoNEBDcmP |
| 4440 | M | M.SflLIN6DcmP (100% identity) | CCWGG | M.EcoNEBDcmP |
| 4445 | phosphohydrolase | |||
| 10980 | DNA-binding transcriptional regulator Fis | |||
| 10985 | M | M.Sso8ORF2545P (100% identity) | ATGCAT | M.EcoNEBORF10985P |
| 10990 | DUF2556 family protein | |||
| 11605 | ribulose-phosphate 3-epimerase | |||
| 11610 | M | M.UbaC1152DamP (100% identity) | GATC | M.EcoNEBDamP |
| 11615 | cell division protein DamX | |||
| 22820 | DUF4060 family protein | |||
| 22825 | M | M.SenHNK130ORF1185P (95% identity) | M.EcoNEBORF22825P | |
| 22830 | DUF1317 family protein | |||
| plasmid pD25466 | ||||
| 160 | 297 aa hypothetical protein | |||
| 165 | M | M.SmaKC004ORF48960P (93% identity) | M.EcoNEBORF165P | |
| 170 | incFII family plasmid replication initiator | |||
| 550 | 73 aa hypothetical protein | |||
| 555 | M | M.SmaBWH35ORF25885P (100% identity) | M.EcoNEBORF555P | |
| 560 | 56 aa hypothetical protein | |||
| Type IV | ||||
| ORF | Gene | Most similar | Specificity | Name |
| Chromosome | ||||
| 16575 | 978 aa hypothetical protein | |||
| 16580 | R | SenHNK130McrCP (100% identity) | EcoNEBMcrCP | |
| 16585 | R | SenHNK130McrBP (100% identity) | EcoNEBMcrBP | |
| 16590 | endoribonuclease SymE | |||
| 16605 | type I restriction-modification system | |||
| 16610 | R | SenHNK130MrrP (100% identity) | EcoNEBMrrP | |
| 16615 | GTPase | |||
| 22280 | site-specific DNA recombinase | |||
| 22285 | R | SsoSE61ORF22640P (100% identity) | YCGR | EcoNEBORF22285P |
| 22290 | isocitrate/isopropylmalate family dehydrogenase | |||